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n methyl d aspartic acid  (MedChemExpress)


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    Structured Review

    MedChemExpress n methyl d aspartic acid
    N Methyl D Aspartic Acid, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n+methyl+d+aspartate/NMDA/pmc13243376-37-5-20
    Average 95 stars, based on 50 article reviews
    n methyl d aspartic acid - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: A TLK2-mediated calcium-driven cell death pathway links neuronal degeneration to nuclear envelope disruption
    Article Snippet: N-methyl-D-aspartate (MedChemExpress, HY-17551) was prepared in PBS.

    In Vitro:

    Article Title: SHMT2 overexpression improves glaucoma by enhancing mitophagy in retinal ganglion cells through promoting the phospho of PINK1.
    Article Snippet: The cells were cultured in RPMI 1640 medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% penicillin/streptomycin, and maintained in an incubator at 37 °C with 5% CO2. .. To simulate the glaucoma environment in vitro, the RGCs were treated with 100 μM N-methyl-D-aspartate (NMDA; MedChemExpress, Monmouth Junction, NJ, USA) for 24 h. .. Cells were seeded in six-well plates at a density of 2 × 105 cells per well and transfected with SHMT2-overexpressing plasmids (pcDNA3.1-SHMT2) or the empty vector (pcDNA3.1) (GenePharma, Shanghai, China) using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA).

    Article Title: SHMT2 overexpression improves glaucoma by enhancing mitophagy in retinal ganglion cells through promoting the phospho of PINK1
    Article Snippet: The cells were cultured in RPMI 1640 medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% penicillin/streptomycin, and maintained in an incubator at 37 °C with 5% CO 2 . .. To simulate the glaucoma environment in vitro, the RGCs were treated with 100 μM N-methyl-D-aspartate (NMDA; MedChemExpress, Monmouth Junction, NJ, USA) for 24 h. .. Cells were seeded in six-well plates at a density of 2 × 10 5 cells per well and transfected with SHMT2-overexpressing plasmids (pcDNA3.1-SHMT2) or the empty vector (pcDNA3.1) (GenePharma, Shanghai, China) using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA).



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    Image Search Results


    ( A ) A brightfield (BF) image of the neuronal culture on day 7, showing established cellular density and neurite outgrowth. Scale bar = 100 μm. ( B ) Immunofluorescence images of the culture at the same time point. From left to right: NfL (green) highlighting axonal structures; DAPI (blue) indicating nuclear localisation; and a merged overlay. Scale bar = 100 μm. ( C ) NfL concentration in the samples, collected at various time points (10 min, 60 min, 24 h) following drug administration, measured by ELISA. Cells were treated with staurosporine (100k cells per well) or NMDA (100k, 150k, 250k cells per well). Data are represented as the mean. All points beyond 400 pg mL −1 of NfL are estimated. ( D ) Tukey’s box plots of the S norm obtained by CV (red, left y -axis), EIS (orange), and CA (blue; both on the right y -axis) for blanks in two matrices—PBS and BP—and three groups of cell culture samples (at low, medium, and high concentration) in BP; separated data points were considered outliers.

    Journal: Biosensors

    Article Title: Electrochemical Detection of Neuronal Injury in Cell Culture Samples: A Cost-Effective Biosensor for Neurofilament Light Sensing

    doi: 10.3390/bios16040212

    Figure Lengend Snippet: ( A ) A brightfield (BF) image of the neuronal culture on day 7, showing established cellular density and neurite outgrowth. Scale bar = 100 μm. ( B ) Immunofluorescence images of the culture at the same time point. From left to right: NfL (green) highlighting axonal structures; DAPI (blue) indicating nuclear localisation; and a merged overlay. Scale bar = 100 μm. ( C ) NfL concentration in the samples, collected at various time points (10 min, 60 min, 24 h) following drug administration, measured by ELISA. Cells were treated with staurosporine (100k cells per well) or NMDA (100k, 150k, 250k cells per well). Data are represented as the mean. All points beyond 400 pg mL −1 of NfL are estimated. ( D ) Tukey’s box plots of the S norm obtained by CV (red, left y -axis), EIS (orange), and CA (blue; both on the right y -axis) for blanks in two matrices—PBS and BP—and three groups of cell culture samples (at low, medium, and high concentration) in BP; separated data points were considered outliers.

    Article Snippet: N-methyl-D-aspartate (NMDA) was obtained from Tocris Bioscience (Bristol, UK).

    Techniques: Immunofluorescence, Concentration Assay, Enzyme-linked Immunosorbent Assay, Cell Culture